Protein Purification
This section covers the expression and purification of the Simiaoa sunii (SsDddA) cytidine deaminase and the DddI inhibitor used in the DAF-seq Protocol.
We recommend storing SsDddA and DddI aliquots at -80 C. Both SsDddA and DddI are stable for at least 5 freeze-thaw cycles.
Addgene
The dual expression plasmid for SsDddA and the expression plasmid for DddI are now avaiable through Addgene!
Need purified protein?
Email absterga@uw.edu for purified SsDddA and DddI protein aliquots.