Protein Purification

This section is under development. Protocols for purifying SsDddA and DddI will be added soon.

Planned content includes:

• Expression and purification of SsDddA (the Simiaoa sunii cytidine deaminase used in the DAF-seq Protocol)
• Expression and purification of DddI (the deaminase inhibitor used to stop the reaction)
• Recommended storage conditions and aliquoting strategies
• Quality control assays for verifying enzyme activity

In the meantime, refer to the methods section of the DAF-seq publication for purification details.