Protein Purification
This section covers the expression and purification of the Simiaoa sunii (SsDddA) cytidine deaminase and the DddI inhibitor used in the DAF-seq Protocol.
We recommend storing SsDddA and DddI aliquots at -80 C. Both SsDddA and DddI are stable for at least 5 freeze-thaw cycles.
Example Targeted DAF-seq deamination rates from multiple batches of SsDddA prepared at two different labs are available for reference here.
Addgene
The dual expression plasmid for SsDddA and the expression plasmid for DddI are now avaiable through Addgene!
Need purified protein?
Email absterga@uw.edu for purified SsDddA and DddI protein aliquots.